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Application Detail (App ID: 10133)

Column: Prodigy™ 5 µm ODS-3 100 Å, LC Column 250 x 4.6 mm, Ea

Part No:00G-4097-E0

Dimensions:250 x 4.6 mm ID

Elution Type:Gradient

Elution A:Acetic acid/water (20:980 v/v)

Elution B:Acetonitrile/mobile phase A

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	100	0
10	83	20	80
11	85	100	0
2	3	100	0
3	15	96	4
4	30	90	10
5	50	85	15
6	60	77	23
7	66	75	25
8	80	70	30
9	80	50	50

Flow Rate:1 mL/min

Col. Temp: 30 °C

Detection: UV-Vis Abs.-Variable Wave.(UV) @ 280 nm (22°C)

Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

Arbutin

Gallic acid

HMF

Catechin

Gentistic acid

Chlorogenic acid

Epicatechin

p-Coumaric acid

Ferulic acid

Rutin

Phloridzin

Step No.	Time (min)	Pct A	Pct B
1	0	100	0
10	83	20	80
11	85	100	0
2	3	100	0
3	15	96	4
4	30	90	10
5	50	85	15
6	60	77	23
7	66	75	25
8	80	70	30
9	80	50	50

Step No.	Time (min)	Pct A	Pct B
1	0	100	0
10	83	20	80
11	85	100	0
2	3	100	0
3	15	96	4
4	30	90	10
5	50	85	15
6	60	77	23
7	66	75	25
8	80	70	30
9	80	50	50

Step No.	Time (min)	Pct A	Pct B
1	0	100	0
10	83	20	80
11	85	100	0
2	3	100	0
3	15	96	4
4	30	90	10
5	50	85	15
6	60	77	23
7	66	75	25
8	80	70	30
9	80	50	50