Search

Featured Chiral HPLC Columns

Lux Chiral Columns

Polysaccharide-Based Chiral HPLC Column
Allows for screening under reversed, polar organic, and normal phase
Ideal for chiral and SFC purifications

Learn More »
Find Pricing »

Recommended Accessories

Sample Collection

HPLC Vials & Vial Kits »
Sample Filtration

LC & GC Syringe Filters »
Column Protection

HPLC Guard Columns »

Application Structure Search

Find Chiral LC Applications in Minutes

Immediately find chiral LC applications using the application structure search web tool. Simply draw the structure of your analytes or key functional groups to quickly and easily find an applicatoion.
Find an application now »

Separate Enantiomers

Chiral Chromatography Webinar HPLC Course

A Quick Look at Chiral HPLC

Chiral chromatography refers to the separation of enantiomers using a chiral HPLC column, an HPLC column that is packed with a chiral stationary phase (CSP). Enantiomers are separated based on the number and type of each interaction that occurs during their exposure to the chiral stationary phase. Some chiral stationary phases (CSPs) are able to separate a wide range of chiral compounds, while others are useful only for specific types of chiral compounds.

Normal phase solvents are commonly used for chiral HPLC separations, however, reversed phase solvents can also be used with certain types of chiral stationary phases. Approximately 60% of all pharmaceutical drugs are chiral. Common chiral stationary phases used for chiral HPLC columns are polysaccharide, ligand exchange, protein, helical polymers, macrocyclic, and Brush (Pirkle-concept).

Chiral HPLC is used to Separate Enantiomers

<link href="//phenomenex.blob.core.windows.net/cdn/cdncss/mgtechniquelc.css" rel="stylesheet" />
<style type="text/css">ul.luxlist {
width:200px;
margin-left:-20px!important;

}

ul.luxlist li {
list-style:none;	
}

ul.luxlist li:before {
    position: absolute;
    content: "";
    border-radius: 50%;
    width: 6px;
    height: 6px;
    background-color: #007f92;
    margin-left: -13px;
    margin-top: 5px;
}
</style>

<section class="hplctechgrid">
<div class="left-top-col-grid">
<div class="searchcard">
<h2> Search</h2>

<ul class="prodsrch">
	<li class="lccolumn-search"><a class="prods" href="/Products/Search/HPLC?mode=Chiral&cookie=false">Chiral LC HPLC/UHPLC Columns</a></li>
	<li class="appcolumn-search"><a class="prods" href="/Application/Search?separationMode=Chiral%20Chromatography&cookie=false">Chiral HPLC/UHPLC Applications</a></li>
	<li class="acccolumn-search"><a class="prods" href="/Products/Search/Accessories">HPLC/UHPLC Accessories</a></li>
</ul>
</div>
</div>

<div class="right-top-col-grid">
<h2 class="featuredheader">Featured Chiral HPLC Columns</h2>

<div class="subright">
<h2><span class="blktitle">Lux Chiral Columns</span></h2>

<ul class="luxlist">
	<li>Polysaccharide-Based Chiral HPLC Column</li>
	<li>Allows for screening under reversed, polar organic, and normal phase</li>
	<li>Ideal for chiral and SFC purifications</li>
</ul>
<a class="btn btn-primary btn-small" href="/Products/HPLCDetail/lux" style="margin-left:5px;"> Learn More »</a><br />
<a class="btn btn-danger btn-small" href="/Products/HPLCDetail/lux?order=true" style="margin-top:10px;margin-left:5px;"> Find Pricing »</a> </div>
</div>
</div>
</div>

<section class="addons">
<h2><span class="gray-header">Recommended Accessories</span></h2>

<ul class="lcaccessories" style="margin-top:-20px;margin-left:-20px">
	<li>
	<h2><span class="acchead">Sample Collection</span></h2>

<div class="imageframe"><a href="/Products/VialDetail/Verex"><img border="0" class="mini-logo" src="//phenomenex.blob.core.windows.net/images/6785d087-4b0c-43cc-b383-847ccddba43d.png" style="left:70px; margin-top:10px" width="75" /></a> <a href="/Products/VialDetail/Verex"><img border="0" class="accimg" height="70" src="/webimage/16-vial-family-250.png" style="margin-top:-12px; padding-left:10px" /></a> </div>
	<a alt="HPLC Vials and Vial Kits" class="lcacclink" href="/Products/VialDetail/Verex">HPLC Vials & Vial Kits »</a></li>
	<li>
	<h2><span class="acchead">Sample Filtration</span></h2>

<div class="imageframe"><a href="/phenex"><img border="0" class="mini-logo" src="//phenomenex.blob.core.windows.net/images/b49e2636-f094-4c64-be8c-310d7e5c6cfb.png" style="left:100px; z-index:10;" width="75" /></a> <a href="/Phenex"><img alt="Syringe Filters" border="0" class="accimg" height="70" src="/webimage/220x105phenex.png" style="margin-top:-12px; margin-left:-20px" /></a> </div>
	<a class="lcacclink" href="/phenex">LC & GC Syringe Filters »</a></li>
	<li>
	<h2><span class="acchead">Column Protection</span></h2>

<div class="imageframe"><a href="/Products/HPLCDetail/SecurityGuard%20Standard"><img border="0" class="mini-logo" src="//phenomenex.blob.core.windows.net/images/b78ccb23-a4d8-4479-8645-e2fd48d7ffb7.png" style="left:80px; z-index:10;" width="75" /></a> <a href="/Products/HPLCDetail/SecurityGuard%20Standard"><img alt="Guard Columns" border="0" class="accimg" height="70" src="/webimage/sgu-top-slide-shot.png" style="margin-top:-12px; margin-left:40px" /></a> </div>
	<a class="lcacclink" href="/Products/HPLCDetail/SecurityGuard%20Standard">HPLC Guard Columns »</a></li>
</ul>
</section>

<section class="methoddev">
<h2><span class="gray-header">Application Structure Search</span></h2>

<div class="toolframe">
<h2><span class="toolheader">Find Chiral LC Applications in Minutes</span></h2>

<ul class="lctool" style="margin-top:42px;">
	<li class="toolico"><a href="/Application/StructureSearch"><img border="0" src="http://www.phenomenex.com/webimage/structureicon.png" /></a></li>
	<li class="tooldetail">
	<p style="padding-right:30px;">Immediately find chiral LC applications using the <a href="/Tools/ColumnMatch">application structure search</a> web tool. Simply draw the structure of your analytes or key functional groups to quickly and easily find an applicatoion.</p>
	<a class="btn btn-small btn-primary" href="/Application/StructureSearch" style="margin-top:10px;">Find an application now »</a></li>
</ul>
</div>
</section>

<section class="techoverview">
<h2><span class="gray-header">Separate Enantiomers </span></h2>

<div class="techintro">
<h2 style="margin-bottom:0px;">A Quick Look at Chiral HPLC</h2>

<p style="margin-top:-5px">Chiral chromatography refers to the separation of enantiomers using a chiral HPLC column, an HPLC column that is packed with a chiral stationary phase (CSP). Enantiomers are separated based on the number and type of each interaction that occurs during their exposure to the chiral stationary phase. Some chiral stationary phases (CSPs) are able to separate a wide range of chiral compounds, while others are useful only for specific types of chiral compounds.</p>
</div>

<p>Normal phase solvents are commonly used for chiral HPLC separations, however, reversed phase solvents can also be used with certain types of chiral stationary phases. Approximately 60% of all pharmaceutical drugs are chiral. Common chiral stationary phases used for chiral HPLC columns are polysaccharide, ligand exchange, protein, helical polymers, macrocyclic, and <a href="/Products/HPLCDetail/Chirex">Brush (Pirkle-concept)</a>.</p>
<img alt="Chiral HPLC is used to Separate Enantiomers" src="/webimage/chiral_enantiomers.png" title="Chiral Chromatography" /> </section>
</section>
<script src="//cdnjs.cloudflare.com/ajax/libs/gsap/latest/TweenMax.min.js"></script><script type="text/javascript" src="//www.phenomenex.com/Scripts/jquery.cycle.all.js"></script><script type="text/javascript" src="//phenomenex.blob.core.windows.net/cdn/cdnscripts/mgtechniquelc.js"></script><script type="text/javascript">
setTimeout(function(){var a=document.createElement("script");
var b=document.getElementsByTagName("script")[0];
a.src=document.location.protocol+"//script.crazyegg.com/pages/scripts/0018/4268.js?"+Math.floor(new Date().getTime()/3600000);
a.async=true;a.type="text/javascript";b.parentNode.insertBefore(a,b)}, 1);
</script>

Applications
All Applications »

Featured

BROCHURE

Affordable Chiral Columns, Brilliant Separation Power

Lux Replaces DAICEL CHIRALCEL and CHIRALPAK Columns at Substantial Cost Savings!
GUIDE

Chiral Column Selection Guide

Pick the right chiral column the first time, every time, RISK-FREE.
TECHNICAL NOTE

Immobilized versus Coated Polysaccharide Chiral HPLC Columns

Performance Evaluation of Immobilized and Coated Polysaccharide Chiral HPLC Columns Using Generic Screening Mobile Phase Systems
POSTER

Chiral HPLC/SFC Method Development Poster

Access our simplified chiral HPLC/SFC column screening strategies.

Search

Featured Chiral Purification Columns

Axial Compression Packed Lux Chiral Preparative Columns

Available in 21.2mm, 30mm, and 50mm IDs
Axial compression allows for extended column lifetime and improved performance
Ideal for large scale chiral and SFC purifications

Learn More »
Find Pricing »

Recommended Accessories

Sample Collection

HPLC Vials & Vial Kits »
Sample Filtration

LC & GC Syringe Filters »
Column Protection

HPLC Guard Columns »

Application Structure Search

Find Chiral LC Applications in Minutes

Immediately find chiral LC applications using the application structure search web tool. Simply draw the structure of your analytes or key functional groups to quickly and easily find an applicatoion.
Find an application now »

Purify Enantiomeric Compounds

A Quick Look at Chiral HPLC/SFC Purification

Chiral purification by chromatography is becoming more popular as a highly efficient and cost-effective solution to purify enantiomeric compounds. With the strict purity requirements, chemists choose chiral purification by chromatography for speedy and reliable results.

Preparative lab groups are under increased pressured by drug discovery and medicinal chemistry groups to quickly and effectively purify their growing number of chiral compounds. Chiral purification by chromatography overcomes these obstacles effectively and can be easily transferred downstream to analytical testing.

Chiral purifications can be achieved through HPLC and SFC techniques. We offer a variety of chiral stationary phases (CSPs) that are availabe in axial packed preparative columns as well as bulk media for larger scale up.

<link href="//phenomenex.blob.core.windows.net/cdn/cdncss/mgtechniquelc.css" rel="stylesheet" />
<style type="text/css">ul.luxlist {
width:200px;
margin-left:-20px!important;

}

ul.luxlist li {
list-style:none;	
}

<ul class="prodsrch">
	<li class="lccolumn-search"><a class="prods" href="/Products/Search/HPLC?mode=Chiral&cookie=false">Chiral HPLC/SFC Columns</a></li>
	<li class="appcolumn-search"><a class="prods" href="/Application/Search?separationMode=Chiral%20Chromatography&cookie=false">Chiral HPLC/SFC Applications</a></li>
	<li class="acccolumn-search"><a class="prods" href="/Products/Search/Accessories">HPLC/UHPLC Accessories</a></li>
</ul>
</div>
</div>

<div class="right-top-col-grid">
<h2 class="featuredheader">Featured Chiral Purification Columns</h2>

<div class="subright">
<h2 style="padding-right:5px;"><span class="blktitle">Axial Compression Packed Lux Chiral Preparative Columns</span></h2>

<ul class="luxlist">
	<li>Available in 21.2mm, 30mm, and 50mm IDs</li>
	<li>Axial compression allows for extended column lifetime and improved performance</li>
	<li>Ideal for large scale chiral and SFC purifications</li>
</ul>
<a class="btn btn-primary btn-small" href="/Products/HPLCDetail/lux" style="margin-left:5px;"> Learn More »</a><br />
<a class="btn btn-danger btn-small" href="/Products/HPLCDetail/lux?order=true" style="margin-top:10px;margin-left:5px;"> Find Pricing »</a> </div>
</div>
</div>
</div>

<section class="addons">
<h2><span class="gray-header">Recommended Accessories</span></h2>

<ul class="lcaccessories" style="margin-top:-20px;margin-left:-20px">
	<li>
	<h2><span class="acchead">Sample Collection</span></h2>

<section class="methoddev">
<h2><span class="gray-header">Application Structure Search</span></h2>

<div class="toolframe">
<h2><span class="toolheader">Find Chiral LC Applications in Minutes</span></h2>

<section class="techoverview">
<h2><span class="gray-header">Purify Enantiomeric Compounds </span></h2>

<div class="techintro">
<h2 style="margin-bottom:0px;">A Quick Look at Chiral HPLC/SFC Purification</h2>

<p style="margin-top:0px">Chiral purification by chromatography is becoming more popular as a highly efficient and cost-effective solution to purify enantiomeric compounds. With the strict purity requirements, chemists choose chiral purification by chromatography for speedy and reliable results.</p>
</div>

<p>Preparative lab groups are under increased pressured by drug discovery and medicinal chemistry groups to quickly and effectively purify their growing number of chiral compounds. Chiral purification by chromatography overcomes these obstacles effectively and can be easily transferred downstream to analytical testing.</p>

<p>Chiral purifications can be achieved through HPLC and SFC techniques. We offer a variety of chiral stationary phases (CSPs) that are availabe in axial packed preparative columns as well as bulk media for larger scale up.</p>
<img alt="Chiral HPLC/SFC Purification using Axia" src="/webimage/chiral_purification_axia.png" /> </section>
</section>
<script src="//cdnjs.cloudflare.com/ajax/libs/gsap/latest/TweenMax.min.js"></script><script type="text/javascript" src="//www.phenomenex.com/Scripts/jquery.cycle.all.js"></script><script type="text/javascript" src="//phenomenex.blob.core.windows.net/cdn/cdnscripts/mgtechniquelc.js"></script><script type="text/javascript">
setTimeout(function(){var a=document.createElement("script");
var b=document.getElementsByTagName("script")[0];
a.src=document.location.protocol+"//script.crazyegg.com/pages/scripts/0018/4268.js?"+Math.floor(new Date().getTime()/3600000);
a.async=true;a.type="text/javascript";b.parentNode.insertBefore(a,b)}, 1);
</script>

Applications
All Applications »

Featured

BROCHURE

Excellent Axia pre-packed preparative HPLC/SFC columns

Available in over 40 unique achiral and chiral selectivities, Axia PREP LC columns offer:

Increased Performance, Groundbreaking Lifetimes, Optimized Loadability, and Increased Reproducibility
TECHNICAL NOTE

Chiral HPLC and SFC Comparison

Direct Comparison of HPLC and SFC for the Milligram to Gram Scale Purification of Enantiomers
TECHNICAL NOTE

Chiral HPLC Purification with Lux AXIA Columns

Purification of Optically Active Pharmaceutical Compounds using Axial Compressed Columns
GUIDE

Chiral Column Selection Guide

Pick the right chiral column the first time, every time, RISK-FREE.

FREE Chiral Screening Services
We Do the Work for You

PhenoLogix Method Development Services consists of a team of scientists who are well versed in all aspects of HPLC method development. Reliable HPLC methods and fast turnarounds are our specialty for Chiral HPLC Analysis. Depending on compound type, we screen multiple chiral stationary phases under different conditions (Normal Phase, Reversed Phase, and Polar Organic) and return a detailed report of the recommended separation method so you can begin work right away.

Learn More About PhenoLogix» Get Started With PhenoLogix»

"We requested two different chiral compounds be separated and purified. We received the first set of compounds at 99 % e.e. within a week and the second set in 2 weeks, also at greater than 99 % e.e. The second set was a difficult separation. We were very happy with the quality of the separation and the quick turnaround, which was tantamount. Jeff Layne and his group did a great job of informing me on the progress and chemistry/separation issues that arose. I have recommended this services to other colleagues in the Pharmaceutical and BioTech industry here in San Diego and I look forward to using them again. "

Isabelle Okuda
Celgene Corporation,
San Diego, California, USA

.screenright h2 {font-size:20px; line-height:22px;}

.screenright p {
 font-size:12px; 
 line-height:16px;
}

.testimonial {width:575px; border-radius:10px; height:200px; padding-top:8px;padding-left:20px; background-color:#eaeaea; opacity:.9;position:relative; padding-right:10px;}

.testimonial:before {content:"";position: absolute; right: 100%; top: 76px;width: 0; height: 0; border-top: 13px solid transparent; border-right: 26px solid #eaeaea; border-bottom: 13px solid transparent;}

.testimonial p {color:#0661aa;}
</style>
<div class="screenleft"><img src="/webimage/phx_chiral_screening_services.png" width="165" /> </div>

<div class="screenright">
<h2>FREE Chiral Screening Services<br />
<span style="color:#000">We Do the Work for You</span></h2>

<p>PhenoLogix Method Development Services consists of a team of scientists who are well versed in all aspects of HPLC method development. Reliable HPLC methods and fast turnarounds are our specialty for Chiral HPLC Analysis. Depending on compound type,<strong> we screen multiple chiral stationary phases under different conditions (Normal Phase, Reversed Phase, and Polar Organic) and return a detailed report</strong> of the recommended separation method so you can begin work right away.</p>
<a class="btn btn-primary btn-large" href="/Home/Phenologix" style="margin-top:10px">Learn More About PhenoLogix»</a> <a class="btn btn-primary btn-large" href="/Services/Phenologix/Chiral" style="margin-top:10px">Get Started With PhenoLogix»</a> </div>

<div class="testimonial">
<p><em>"We requested two different chiral compounds be separated and purified. We received the first set of compounds at 99 % e.e. within a week and the second set in 2 weeks, also at greater than 99 % e.e. The second set was a difficult separation. We were very happy with the quality of the separation and the quick turnaround, which was tantamount. Jeff Layne and his group did a great job of informing me on the progress and chemistry/separation issues that arose. I have recommended this services to other colleagues in the Pharmaceutical and BioTech industry here in San Diego and I look forward to using them again. "</em><br />
<br />
Isabelle Okuda<br />
Celgene Corporation,<br />
San Diego, California, USA</p>
</div>

Applications
All Applications »

Chiral HPLC Columns

Search

Featured Chiral HPLC Columns

Lux Chiral Columns

Recommended Accessories

Sample Collection

Sample Filtration

Column Protection

Application Structure Search

Find Chiral LC Applications in Minutes

Separate Enantiomers

A Quick Look at Chiral HPLC

Featured

Search

Featured Chiral Purification Columns

Axial Compression Packed Lux Chiral Preparative Columns

Recommended Accessories

Sample Collection

Sample Filtration

Column Protection

Application Structure Search

Find Chiral LC Applications in Minutes

Purify Enantiomeric Compounds

A Quick Look at Chiral HPLC/SFC Purification

Featured

FREE Chiral Screening Services We Do the Work for You

FREE Chiral Screening Services
We Do the Work for You