Phree Phospholipid Removal Information

Phree Phospholipid Removal Solutions offer a fast effective way to remove proteins AND phospholipids from plasma samples without negatively affecting the recovery of your target analytes. The Solvent Shielding Technology™ design of the Phree Phospholipid Removal products will withhold organic solvents above the filter membranes for up to 25 minutes, allowing for direct in-well or in-tube precipitation upon the addition of plasma. Once precipitation is complete, the sample is filtered through the Phree sorbent which selectively removes phospholipids, resulting in a protein and phospholipid free sample.

One Quick Method, Four Big Advantages

Avoid high back pressures by removing proteins that can clog HPLC/UHPLC columns
Extend HPLC/UHPLC column lifetime by removing phospholipids that can build up on your column
Eliminate ion-suppression by removing phospholipids
Skip the method development; one method for acids, bases, and neutrals
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Is Your LC/MS/MS Suffering from Phospholipiditis?

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<p style="cursor: auto;"><strong style="cursor: auto;">One Quick Method, Four Big Advantages</strong></p>

<div style="width: 480px; margin-top: 5px; margin-left: 15px; float: left;">
<ol style="margin-left: 35px;">
	<li style="padding-top: 11px;">Avoid high back pressures by removing proteins that can clog HPLC/UHPLC columns</li>
	<li style="padding-top: 11px;">Extend HPLC/UHPLC column lifetime by removing phospholipids that can build up on your column</li>
	<li style="padding-top: 11px;">Eliminate ion-suppression by removing phospholipids</li>
	<li style="padding-top: 11px;">Skip the method development; one method for acids, bases, and neutrals</li>
	<li><a class="btn btn-primary" href="/home/video/phreedemo.mp4" style="margin-top:10px">Play Video »</a></li>
</ol>
</div>

<p style="cursor: auto;"><strong style="cursor: auto;">Is Your LC/MS/MS Suffering from Phospholipiditis?</strong></p>

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	<li style="cursor: auto;"><a href="/Info/WebDocumentServe/phospholipid_ruin_samples_info_graphic.pdf" style="cursor: pointer;">View our infographic and find out »</a></li>
	<li style="cursor: auto;"><a href="https://phenomenex.blob.core.windows.net/documents/7fec3aae-7ca1-488b-bc07-34472915b885.pdf" style="cursor: pointer;" title="Phree FAQ Sheet">View the FAQ sheet to learn more »</a></li>
</ul>

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Featured

BROCHURE

Advance Your Protein Precipitation with Phree Phospholipid Removal Solutions

Learn how to instantly improve your LC/MS/MS analysis without additional method development.
TECHNICAL NOTE

Rapid Improvements for LC/MS/MS Analysis without Additional Method Development

By following the same protocol as a protein precipitation, you can instantly improve your LC/MS/MS analysis by using Phree Phospholipid Removal 96-well plates and tubes.
VIDEO

Advanced Protein Precipitation and Improved LC/MS/MS Analysis using Phree

View our short video demonstration of the many improvements you can make to your LC/MS/MS analysis by using Phree.
TECHNICAL NOTE

Improved Cleanup of Bioanalytical Samples as Compared to SPE

This technical note describes the benefits that can be realized by performing phospholipid removal as compared to SPE.

Technical Documents
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Document Type	Title
Brochures	Labs Across the World are Advancing their Protein Precipitation	Add to My Documents
Supporting Docs	Clinical Resources for Toxicology Analysis	Add to My Documents
Technical Notes	Determination and Separation of Iohexol in Human Serum by LC-MS/MS using a Kinetex 2.6 μm Polar C18 Core-Shell Column	Add to My Documents
Brochures	Improve Your Bioanalytical Assays Today	Add to My Documents
Webinar	Improved LC/MS/MS Analysis by Eliminating Ion Suppressing Phospholipids	Add to My Documents
Other	Introduction to Phree Phospholipid Removal Products	Add to My Documents
Technical Notes	LC-MS/MS-based Quantification of 47 Therapeutic Drug Monitoring Compounds in Serum: A Simple Sample Preparation Strategy for Efficient Analysis (TN-0159)	Add to My Documents
Other	Method Development Webinar Poster	Add to My Documents
Webinar	Overcoming the Challenges of Sample Preparation and LC/MS/MS Method Development for Clinical Applications	Add to My Documents
Brochures	Phree Phospholipid Removal Solutions	Add to My Documents
Brochures	Presston 1000 Postive Pressure Manifold	Add to My Documents
Technical Notes	Quantitative Analysis of Illicit Drugs of Abuse in Whole Blood by LC-MS/MS	Add to My Documents
Brochures	Sample Preparation Accessories	Add to My Documents
Other	Sample Preparation Reference Manual & Users Guide	Add to My Documents
Webinar	How to Determine the Best Sample Preparation Technique for your Unique Samples	Add to My Documents
Other	THE Clinical Application Notebook, Volume 2	Add to My Documents
Technical Notes	25-OH Vitamin D2 and D3 from Serum	Add to My Documents
Technical Notes	A Rapid, Automatable Solution for Phosphatidylethanol (PEth) LC-MS/MS Analysis in Whole Blood	Add to My Documents
Technical Notes	A Simpler, Faster Solution to Bioanalytical Sample Cleanup (TN-0065)	Add to My Documents
Technical Notes	A Unified Sample Preparation Procedure for General Unknown Screening (GUS) of Compounds in Whole Blood Samples	Add to My Documents
Supporting Docs	An Improved Method for Eliminating Ion Suppression and Other Phospholipid-Induced Interferences in Bioanalytical Samples	Add to My Documents
Technical Notes	Clean-up and Analysis of 12 Cannabinoids from Whole Blood using LC-MS/MS (TN-0130)	Add to My Documents
Technical Notes	Comparison of Two Clean up Techniques and a Quick Analysis of 39 Pain Management Drugs from Serum (AN-1019)	Add to My Documents
Technical Notes	Fast Clean Up of Whole Blood Using Phree Phospholipid Removal and Analysis of 39 Pain Management Drugs by Kinetex 2.6 µm Biphenyl (AN-1018)	Add to My Documents
Technical Notes	Fully Automated Sample Preparation for the Determination of Vitamin D Using Phree Phospholipid Removal	Add to My Documents
Supporting Docs	How to Improve Sample Preparation Accuracy, Reproducibility, and Lab-to-Lab Variation with Automation	Add to My Documents
Technical Notes	Metformin from Human Plasma by Phospholipid Removal and LC-MS/MS (TN-1243)	Add to My Documents
Brochures	Protect Your Mass Spec: Sample Preparation Solutions for Biological Samples	Add to My Documents
Technical Notes	Vitamin D2 and D3 from Human Plasma by LC/MS/MS (TN-0061)	Add to My Documents
Technical Notes	Rapid Improvements for LC/MS/MS Analysis without Additional Method Development (TN-0055)	Add to My Documents
Brochures	A Simpler, Easier Solution to Bioanalytical Sample Preparation as Compared to SPE	Add to My Documents
Supporting Docs	Collection Plate and Sealing Mat Selection Guide	Add to My Documents
Other	Elimination of Suppression Effects in LC/MS/ MS Analysis Using Plate Based Phospholipid Removal Methods	Add to My Documents
Technical Notes	Maximizing Analyte Recoveries using Phree Phospholipid Removal Products (TN-0058)	Add to My Documents
Technical Notes	Maximizing Recovery of 25-OH Vitamin D2 and D3 using Phospholipid Removal Plates and Core-Shel LC-MS/MS Analysis (TN-0070)	Add to My Documents
Other	Optimizing the Effectiveness of Plasma Protein Precipitation using the Direct Addition Technique	Add to My Documents
Supporting Docs	Phree Phospholipid Removal FAQ	Add to My Documents
Supporting Docs	SPE 10, 12, and 24 Position Vacuum Manifold Instructions	Add to My Documents
Supporting Docs	SPE 96-Well Vacuum Manifold	Add to My Documents
Supporting Docs	Declaration of Conformity	Add to My Documents
Supporting Docs	Phree User Guide	Add to My Documents

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Phree Phospholipid Removal Solutions

Instantly eliminate ion suppression and increase column lifetime

Phree Phospholipid Removal Information

Featured

Advance Your Protein Precipitation with Phree Phospholipid Removal Solutions

Rapid Improvements for LC/MS/MS Analysis without Additional Method Development

Advanced Protein Precipitation and Improved LC/MS/MS Analysis using Phree

Improved Cleanup of Bioanalytical Samples as Compared to SPE