UHPLC/HPLC Method Translator

Welcome to your UHPLC/HPLC Method Transfer Tool!

As you develop a new method or take on an existing method, it can be time consuming to figure out the exact adjustments needed to transfer said method across different instrumentation, particle morphologies or even LC particle sizes. We've definitely felt that pain over the years and created this Method Transfer Tool to allow you to take back that time!

Enjoy!

Need a column recommendation? Ask Us!


LC Instrument 1		Predicted Total Pressure (bar)	LC Instrument 2		Predicted Total Pressure (bar)
Pressure Limit (bar) =		0	Pressure Limit (bar) =
Delay Volume (mL) =	Typical Delay Volume Values: 1.100 mL Agilent 1100/1260 Binary 1.000 mL Agilent 1100/1260 Quaternary 0.450 mL Agilent 1290 Quaternary 0.450 mL Waters ACQUITY H-Class 0.200 mL Agilent 1290 Binary 0.125 mL Waters ACQUITY UPLC/I-Class Close		Delay Volume (mL) =	Typical Delay Volume Values: 1.100 mL Agilent 1100/1260 Binary 1.000 mL Agilent 1100/1260 Quaternary 0.450 mL Agilent 1290 Quaternary 0.450 mL Waters ACQUITY H-Class 0.200 mL Agilent 1290 Binary 0.125 mL Waters ACQUITY UPLC/I-Class Close
Instrument Type =			Instrument Type =
Maximum Viscosity =		0	Separation Goal =


LC Column 1					LC Column 2
Length (mm)	Column ID (mm)	Flow Rate (mL/min)	Particle Type / Size (μm)	Porosity	Length (mm)	Column ID (mm)	Flow Rate (mL/min)	Particle Type / Size (μm)	Porosity
			5 μm Core-Shell 5 μm Fully Porous 4 μm Fully Porous 3.6 μm Core-Shell 3.5 μm Fully Porous 3 μm Fully Porous 2.6 μm Core-Shell 1.7 μm Core-Shell 1.6 μm Fully Porous 1.3 μm Core-Shell Close	0			0	5 μm Core-Shell 5 μm Fully Porous 4 μm Fully Porous 3.6 μm Core-Shell 3.5 μm Fully Porous 3 μm Fully Porous 2.6 μm Core-Shell 1.7 μm Core-Shell 1.6 μm Fully Porous 1.3 μm Core-Shell Close	0
Original Gradient Table					Scaled Gradient Table
Time (min)	%A	%B	#CV	%B/CV	Time	%A	%B	#CV	%B/CV
0		0	0		0	0	0	0
		0	0		0	0	0	0	0
		0	0	0	0	0	0	0	0
		0	0	0	0	0	0	0	0
		0	0	0	0	0	0	0	0
		0	0	0	0	0	0	0	0
		0	0	0	0	0	0	0	0


1 Column Volume (mL) =	0	1 Column Volume (mL) =	0
Min Equilibration Time (min) =	0	Min Equilibration Time (min) =	0
Delay Time (min) =	0	Delay Time (min) =	0
Void Time (min) =	0	Void Time (min) =	0
Linear Velocity (mm/s) =	0	Linear Velocity (mm/s) =	0
		Max Instrument Delay Volume (mL) =	0
		Injection Delay Time (min) =	0