HPLC column and UHPLC Column Performance Kinetex Calculator from Phenomenex

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Kinetex 2.6 µm Calculator

Enter your current system, column, and method details below. The Kinetex 2.6 µm Calculator will use this information to determine the best Kinetex column for you along with increases in efficiency, resolution, and productivity. You will also see the potential cost savings by switching your method to Kinetex.

Current System

System Manufacturer

System Model

System Data

Max allowable flow rate

(mL/min)

Max allowable pressure

(Bar)

Current Column From list Manual input

Column supplier

Column brand

Column phase

Column length (mm)

Column ID (mm)

Particle size (µm)

Current Method Information

Efficiency (plates/column)

Maximum efficiency: 100000

Resolution of critical pair

Maximum resolution: 50

Observed backpressure ( Bar or PSI )

Total run time (minutes)

Example: 23.05

Mode of Operation isocratic gradient

Flow rate (mL/min)

Maximum flow rate:

Injection volume (µL)

Maximum injection volume: 100

Step No. #	Time (min)	Flow Rate (ml/min)	% Solvent (%) A	% Solvent (%) B	% Solvent (%) C	% Solvent (%) D
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Injection volume (µL)

Maximum injection volume: 100

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Kinetex Phase Selection

C18

Balanced C18 phase that provides the highest degree of hydrophobic selectivity relative to the other Kinetex phases.

XB-C18

Iso-butyl side chains make this C18 unique, resulting in improved peak shape for basic compounds and increased retention of acidic compounds.

Moderate hydrophobic and steric selectivity is offered, bringing ultra-high performance to USP L7 and other octyl silane methods.

Phenyl-Hexyl

Aromatic and moderate hydrophobic selectivity results in the great retention and separation of aromatic hydrocarbons.

HILIC

Used under HILIC running conditions, this unbonded silica phase provides the highest polar selectivity for retention and separation of hydrophilic compounds.

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