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| Component | As per USP | Adjustable from | Adjustable to | Limit |
|---|---|---|---|---|
| Mobile Phase Minor Component (<50%) | ±30% relative; cannot exceed ±10% absolute adjustment; Cannot be reduced to zero | |||
| Mobile Phase pH | ±0.2 units | |||
| Buffer Concentration (mM) | ±10% | |||
| Column Temperature (°C) | ±10°C | |||
| Flow Rate (mL/min) | ±50% (at same ID) |
| As per USP | Desired column dimensions | |||
| Column Length (mm) | ||||
| Column Diameter (mm) | ||||
| Particle Size (µm) | *Alternatively (as for the application of particle size adjustment to superficially porous particles), other L/dp combinations can be used provided that the number of theoretical plates (N) is within -25% to +50% | |||
| L/Dp | % deviated | |||
| The column with desired dimension | ||||
| Relative Flow Rate Suggestion | ||||
| Injection Volume (µL) | This is just a recommendation Can be adjusted as much as needed; must be consistent with linearity, precision, and detection requirements |
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| Stationary Phase | No changes of the identity of the substituent recommended. |
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Choose Phenomenex columns for your analysis |
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| Choose USP column listing | ||||