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Application Detail (App ID: 10257)

Column: Prodigy™ 5 µm Phenyl-3 (PH-3) 100 Å, LC Column 150 x 4.6 mm, Ea

Part No:00F-4298-E0

Dimensions:150 x 4.6 mm ID

Elution Type:Isocratic

Elution A:Acetonitrile

Elution B:20 mM Potassium phosphate pH 3.0

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	25	75

Flow Rate:1.5 mL/min

Col. Temp: 40 °C

Detection: UV-Vis Abs.-Variable Wave.(UV) @ 230 nm (22°C)

Detector Info: Variable wavelength UV detector was changed during the run as follows:
0 to 1.6 min UV @ 230 nm
1.6 to to 2 min UV @ 210 nm
>2 min UV @ 230 nm

Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Phenyl 4 x 3.0mm, 10/Pk Part No.: AJ0-4351
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

Saccharin

Aspartame

p-Hydroxybenzoic acid

Caffeine

Benzoic acid

p-Hydroxybenzoic acid methyl ester

p-Toluic acid

Dehydroacetic acid

p-Hydroxybenzoic acid ethyl ester

p-Hydroxybenzoic acid propyl ester