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Amino Acids

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Application Detail (App ID: 1314)

Column: Luna® 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Part No:00F-4251-E0

Dimensions:150 x 4.6 mm ID

Elution Type:Gradient

Elution A:4 mM CH3COO-Na+, 4% N,N-Dimethylformamide, pH 6.5 adjusted with acetic acid

Elution B:Acetonitrile

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	80	20
2	20	60	40
3	28	30	70
4	38	30	70
5	40	80	20

Flow Rate:0.75 mL/min

Col. Temp: 40 °C

Detection: UV-Vis Abs.-Variable Wave.(UV) @ 436 nm (22°C)

Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

Citrulline

Arginine

Alanine

Proline

Valine

Methionine

Isoleucine

Leucine

Tryptophan

Phenylalanine

unknown

Tyrosine

Nitrotyrosine