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Mobile phases pH vs Selectivity in RP HPLC

Application Detail (App ID: 15015)

Part No:00F-4435-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:Acetonitrile
Elution B:buffers at various pH's (see chromatograms)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 50 50

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 230 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 3.0mm, 10/Pk Part No.: AJ0-7597
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

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