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OPA Amino Acids on Gemini C18

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Application Detail (App ID: 15992)

Column: Gemini® 5 µm C18 110 Å, LC Column 150 x 4.6 mm, Ea

Part No:00F-4435-E0

Dimensions:150 x 4.6 mm ID

Elution Type:Gradient

Elution A:40mM NaH2PO4, pH 7.8

Elution B:Acetonitrile/Methanol/Water (45:45:10)

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	100	0
2	40.5	59.5	40.5
3	41	39	61
4	43	39	61
5	44	18	82
6	44.5	0	100
7	46.5	0	100
8	47	100	0
9	49	100	0

Flow Rate:1 mL/min

Col. Temp: 40 °C

Detection: Fluorimetric @ 340 nm (22°C)

Detector Info: OPA derivatization performed on-line prior to injection on HP1100

Note:Courtesy of Jens Olaf Kroemer, Technische Biochemie, Universität des Saarlandes, Germany

Analyte Details

Analyte Detail not available

Aspartic acid

Glutamic acid

Cysteine

Asparagine

Serine

Homocysteine

Glutamine

Histidine

Homoserin

Glycine

Threonine

Citrulline

Arginine

beta-Alanine

Alanine

gamma-Aminobutyric acid

o-Acetyl-serine

Tyrosine

o-Acetyl-homoserine

alpha-Aminobutyric acid

L-Cystathionine

Histidine 2

Valine

Methionine

Norvaline

Tryptophan

Phenylalanine

Isoleucine

Ornithine

Leucine

Lysine

Proline