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Column Diameter Study with Luna 5u C18(2) 50 x 4.6mm

Application Detail (App ID: 18062)

Part No:00B-4252-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.5% TFA in Acetonitrile
Elution B:0.5% TFA in Water
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 5 95
2 5 95 5

Flow Rate:1.5 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)
Note:Comparision of column diameter on preparative separations using Axia-packed columns in various diameters: 4.6 (conventional hardware), 21.2, 30 and 50mm (cf: App IDs: 18062 - 18065).
Load varied by diameter as follows:
- 4.6mm: 64 µg in 21 µL DMSO (scaling factor = 1)
- 21.2mm: 1.2 mg in 420 µL DMSO (scaling factor = 20)
- 30mm: 2.4 mg in 840 µL DMSO (scaling factor = 40)
- 50mm: 7.3 mg in 2100 µL DMSO (scaling factor = 118)

Analyte Details

Analyte Detail not available

1
Propranolol
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2
Diphenhydramine
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