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Ion Pairing & LC/MS Influence of Oligonucleotides (Figure 6d)

Application Detail (App ID: 18071)

Part No:00D-4441-B0
Dimensions:100 x 2 mm ID
Elution Type:Gradient
Elution A:200mM HFIP/14mMTEA/2% MeOH
Elution B:200mM HFIP/14mMTEA/98% MeOH
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 5 90 10
3 10 85 15
4 60 70 30

Flow Rate:0.25 mL/min
Col. Temp: 50 °C
Detection: Mass Spectrometer (MS) @ m/z (365°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Clarity Oligo-RP 4 x 2.0mm, 10/Pk Part No.: AJ0-8134
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
poly dT (12-18) ladder
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