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Calibration Curve for Melamine Extraction (20-2000ng/mL range) from Strata Melamine on Luna HILIC

Application Detail (App ID: 18467)

Part No:00D-4449-B0
Dimensions:100 x 2 mm ID
Elution Type:Isocratic
Elution A:90/10 ACN/100 mM Ammonium Formate, pH 3.2
Gradient Profile:
Step No. Time (min) Pct A
1 3.5 100

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ (22°C)
Detector Info: * MS in -ve ionization till 1.7 mins then to +ve till 3.5 mins
Note:LC/MS/MS Condition:
Column: Luna 3µm HILIC, 100x2.0mm; Flow rate: 0.4ml/min
Mobile Phase: Isocratic; 90/10 ACN/100mM NH4 Formate pH 3.2; MS Detector: API 3000 LC/MS/MS with ESI (TurboIonSpray®) (www.Appliedbiosystems.com), Switch from -ve Ion Mode (for cyanuric) to Positive Ion (for Melamine);
MRM; heater gas flow 7000 cc/min; heater temperature 450 °C


Melamine QC - 200 ng
% Accuracy 99.45%
% CV 6%


Calibration Curve of Extracted Samples

conc. (ng) % Accuracy
20 102%
40 96%
200 104%
400 98%
2000 100.03%

Analyte Details

Analyte Detail not available

1
Melamine
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2
Melamine 13C3-Amino 15N3
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