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Extraction of Healthcare Opiates using strata X-C on Kinetex 2.6µm C18 50 x 2.1mm ID by LC/MS

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Application Detail (App ID: 18716)

Column: Kinetex® 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Part No:00B-4462-AN

Dimensions:50 x 2.1 mm ID

Elution Type:Gradient

Elution A:0.1% Formic acid and 5 mM ammonium acetate in H2O

Elution B:0.1% Formic acid and 5 mM ammonium acetate in 50-50ACN-Methanol

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	95	5
2	0.24	95	5
3	3.36	20	80
4	3.37	5	95
5	3.68	5	95
6	3.69	95	5
7	5.55	95	5

Flow Rate:400 µL/min

Col. Temp: ambient

Detection: Mass Spectrometer (MS) @ amu (22°C)

Analyte Details

Analyte Detail not available

Normorphine

Morphine

Morphine-d3

Oxymorphone

Hydromorphone

Hydromorphone-d6

Codeine-d6

Codeine

O-Desmethyltramadol

Oxycodone-d6

Oxycodone

Hydrocodone

N-Desmethyltramadol

Tramadol

Normeperidine

Normeperidine-d4

Meperidine-d4

Meperidine