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Extraction of Steroids Using Strata-X on Kinetex 2.6µm C18 50 x 2.1mm ID by LCMS

Application Detail (App ID: 18848)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1%FA in H2O
Elution B:0.1%FA in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 80 20
2 2.5 20 80
3 2.51 5 95
4 2.8 5 95
5 2.81 80 20
6 4.6 80 20

Flow Rate:450 µL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: ESI conditions
Positive Ionization
NEB: 10.00
CUR: 8.00
IS: 4500.00
TEM: 425.00
CAD: 7.00
EP 10.00

Analyte Details

Analyte Detail not available

1
Triamcinolone
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2
Prednisolone
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3
Cortisone
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4
Betamethasone
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5
Corticosterone
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6
Triamcinolone acetonide
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7
11-alpha-Hydroxyprogesterone
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8
Cortisone acetate
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9
Testosterone
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10
11-Ketoprogesterone
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11
Betamethasone 17-valerate
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