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25-OH Vitamin D2 and D3 Control from Serum on Kinetex 2.6µm C18 after PPT

Application Detail (App ID: 19314)

Part No:00B-4462-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.05 % Formic acid
Elution B:5 mM Ammonium acetate + 0.1 % Formic acid in methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 92 8
2 0.08 92 8
3 3.4 0 100
4 4.8 0 100
5 6 92 8

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: SCIEX API 4000™

Analyte Details

Analyte Detail not available

1
25-Hydroxy vitamin D3 (25-OH D3)
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2
25-Hydroxy vitamin D3-d6 (25-OH D3-d6)
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3
25-Hydroxy vitamin D2 (25-OH D2)
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