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25-OH Vitamin D2 and D3 Standards from Serum on Fully Porous 5µm C18 after PPT

Application Detail (App ID: 19315)

Part No:00B-4252-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:0.05 % Formic acid
Elution B:5 mM Ammonium acetate + 0.1 % Formic acid in methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 92 8
2 5 92 8
3 205 0 100
4 290 0 100
5 360 92 8

Flow Rate:0.9 mL/min
Col. Temp: 35 °C
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: SCIEX API 4000™
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-4286
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
25-Hydroxy vitamin D2 (25-OH D2)
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2
25-Hydroxy vitamin D3-d6 (25-OH D3-d6)
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3
25-Hydroxy vitamin D2 (25-OH D2)
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