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Bivalirudin Purification Elution Profile-35mg Load on Column

Application Detail (App ID: 19462)

Part No:00G-4323-E0
Dimensions:250 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% TFA in Water
Elution B:0.1% TFA in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 5 85 15
2 5.01 85 15
3 45 65 35
4 45.01 20 80
5 50 20 80
6 50.01 85 15
7 60 85 15

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Diode Array (PDA) @ 280 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C8 4 x 3.0mm ID, 10/Pk Part No.: AJ0-4290
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Bivalirudin
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