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Testosterone from Female Human Plasma by LC/MS/MS using Strata-X-A and Kinetex 1.7 µm C18

Sample Preparation Details

Part Number :8B-S123-TBJ
Description :Strata-X-A 33 µm Polymeric Strong Anion, 30 mg / 3 mL, Tubes, 50/Pk
Condition 1 :2 mL Methanol
Condition 2 :2 mL Water
Load :Load 1.25 mL Sample diluted in Water
Wash 1 :0.6 mL 50% Methanol in Water
Dry :Dry for 5 min under high vacuum
Elution 1 :0.3 mL Methanol
Elution 2 :0.3 mL Methanol
Evaporate: :Evaporate to dryness
Reconstitute :250 µL 200 uL 5% aqueous formic acid
Notes :Following evaporation of elution solvent @ 50-55 C under gentle nitrogen stream; Add 50 uL 25% hydroxylamine solution and heat at 60-65 C for 5-10 min, then add 200 uL 5% aqueous formic acid and vortex the tubes Transfer the solution to autosampler vials and inject 25 uL on column

Application Detail (App ID: 19762)

Part No:00A-4475-AN
Dimensions:30 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid +1 mM Amm Formate in Water
Elution B:0.1% Formic Acid +1 mM Amm Formate in ACN
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 2.5 10 90
3 3.5 10 90
4 3.6 90 10

Flow Rate:0.4 mL/min
Col. Temp: ambient
Detection: Mass Spectrometer (MS) @ amu (22°C)
Detector Info: SCIEX API 5000™

Analyte Details

Analyte Detail not available

1
Testosterone
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2
Testosterone-d3
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