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Estrogen Mix using Kinetex 1.7u XB-C18 50x2.10mm on Water Acquity UPLC system

Application Detail (App ID: 19799)

Part No:00B-4498-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:6.9mM Ammonium Formate pH 4.0 (v/v) 25% Acetonitrile
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 100 0
2 5 35 65

Flow Rate:0.5 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 254 nm (22°C)

Analyte Details

Analyte Detail not available

1
Estriol
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2
17-beta-Estradiol
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3
Estrone
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