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Hi Temp Stability - Injection on new Aeris WIDEPORE column

Application Detail (App ID: 19832)

Part No:00B-4482-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:Water with 0.1% TFA
Elution B:Acetonitrile with 0.1% TFA
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 97 3
2 3 97 3
3 23 35 65

Flow Rate:0.312 mL/min
Col. Temp: 90 °C
Detection: UV-Vis Abs.-Diode Array (PDA) @ 214 nm (22°C)
Note:Application was actually run on a 50x4.6mm ID running at a flow rate of 1.5 mL/min. 50 x 4.6 mm which is not a commercially available part number. Information switched to 50x2.1mm for those that wish to replicate method.

Analyte Details

Analyte Detail not available

1
Apomyoglobin
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