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Alfatoxins in Food by LC/MS/MS

Sample Preparation Details

Part Number :8B-S013-HBJ
Description :Strata FL-PR Florisil (170 µm, 80 Å), 500 mg / 3 mL, Tubes, 50/Pk
Condition 1 :3 mL Methanol
Condition 2 :3 mL Methanol
Load :Load 1.50 mL Sample
Wash 1 :3 mL Methanol/Water (80:20)
Wash 2 :3 mL Methanol/Water (80:20)
Wash 3 :6 mL Methanol
Elution 1 :3 mL Acetone/Water/Formic Acid (96:3.5:0.5)
Elution 2 :3 mL Acetone/Water/Formic Acid (96:3.5:0.5)
Evaporate: :Evaporate to dryness
Reconstitute :1 mL Mobile Phase

Application Detail (App ID: 20016)

Part No:00B-4447-B0
Dimensions:50 x 2 mm ID
Elution Type:Isocratic
Elution A:5mM Ammonium Acetate + 0.1% Formic Acid
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 40 60

Flow Rate:0.4 mL/min
Col. Temp: 28 °C
Detection: Tandem Mass Spec (MS-MS) @ (0°C)
Detector Info: 3200 QTRAP
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, PFP 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8326
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Aflatoxin M1
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2
Aflatoxin G2
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3
Aflatoxin G1
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4
Aflatoxin B2
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5
Aflatoxin B1
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