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Penicillins in Meat by LC/MS/MS

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Sample Preparation Details

Part Number :8B-S001-FBJ

Description :Strata C18-E (55 µm, 70 Å), 200 mg / 3 mL, Tubes, 50/Pk

Condition 1 :4 mL Methanol at 30 µL/min

Condition 2 :4 mL Water at 30 µL/min

Load :Load 2.00 mL Sample diluted in Filtrate

Wash 1 :4 mL 0.1% Formic Acid in water

Elution 1 :2 mL Acetonitrile at 30 µL/min

Elution 2 :2 mL Acetonitrile at 30 µL/min

Evaporate: :Evaporate to dryness

Reconstitute :200 µL Water

Notes :The equilibration time at conditon 0 was 4 minutes before each injection.

Application Detail (App ID: 20028)

Column: Gemini® 3 µm C18 110 Å, LC Column 150 x 4.6 mm, Ea

Part No:00F-4439-E0

Dimensions:150 x 4.6 mm ID

Elution Type:Gradient

Elution A:0.1% Ammonia in water

Elution B:95% Acetronitrile, 5% water and 0.1% ammonia

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	98	2
2	2	98	2
3	2.42	90	10
4	6	50	50
5	6.1	0	100
6	6.6	0	100
7	6.8	98	2
8	8	98	2

Flow Rate:0.8 mL/min

Col. Temp: 40 °C

Detection: Tandem Mass Spec (MS-MS) @ -4500 V (600°C)

Detector Info: 3200 QTRAP

Note:This separation uses IMethod Kit Part Number KH0-9047

SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-7597
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

Amoxicillin

Ampicillin

Penicillin G

Penicillin V

Oxacillin

Cloxacillin

Nafcillin

Dicloxacillin