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Applications

Tetracyclines in Meat by LC/MS/MS

Sample Preparation Details

Part Number :8B-S100-UBJ
Description :Strata-X 33 µm Polymeric Reversed Phase, 60 mg / 3 mL, Tubes, 50/Pk
Condition 1 :4 mL Methanol at 30 µL/min
Condition 2 :4 mL 0.1% Formic Acid at 30 µL/min
Load :Load 4.00 mL Sample diluted in Filtrate
Wash 1 :2 mL 0.1% Formic Acid at 30 µL/min
Wash 2 :1
Dry :Evaporate to dryness using an eppendorf concentrator set at 45C or blow dry with nitrogen.
Elution 1 :1 mL Methanol
Evaporate: :Evaporate to dryness

Application Detail (App ID: 20032)

Part No:00B-4435-E0
Dimensions:50 x 4.6 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in Water
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 98 2
2 2 98 2
3 2.1 85 15
4 6.5 50 50
5 7.1 0 100
6 8 0 100
7 8.1 98 2
8 10 98 2

Flow Rate:0.8 mL/min
Col. Temp: 40 °C
Detection: Tandem Mass Spec (MS-MS) @ (600°C)
Detector Info: 3200 QTRAP LC/MS/MS
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-7597
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Oxytetracycline
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2
Tetracycline
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3
Chlortetracycline
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4
Methacycline
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5
Doxycycline
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