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Multiclass Antibiotics Screening of Kidney Juice, Serum, Milk and Honey by LC/MS/MS

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Application Detail (App ID: 20033)

Column: Gemini® 3 µm C18 110 Å, LC Column 50 x 2 mm, Ea

Part No:00B-4439-B0

Dimensions:50 x 2 mm ID

Elution Type:Gradient

Elution A:Water + 0.1% Formic Acid

Elution B:Methanol + 0.1% Formic Acid

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	98	2
2	7.27	20	80
3	7.37	1	99
4	11	98	2
5	15	98	2
6	11	98	2
7	15	98	2

Flow Rate:0.5 mL/min

Col. Temp: 40 °C

Detection: Tandem Mass Spec (MS-MS) @ (600°C)

Detector Info: SCIEX 4000 QTRAP®

Note:Sample Prep Steps:

1. Weigh 1 g of homogenized beef kidney sample, kidney juice, or serum into a 50 mL FEP (fluorinated ethylene propylene) tube. Alternatively you can use a disposable polypropylene Corning tube.
2. Add 5 µL of the internal standard work solution.
3. Add 2 mL of water and 8 mL of acetonitrile
4. Mix briefly using a vortex mixer, and then shake for five minutes.
5. Centrifuge at 3450 rcf for five minutes.
6. Decant the supernatant into a 50 mL tube with 500 mg of Sepra C18-E sorbent.
7. Mix briefly using a vortex mixer and shake for 30 seconds.
8. Centrifuge at 3450 rcf for one minute.
9. Place a 5 mL aliquot of the supernatant into a graduated tube.
10. Evaporate down to less than 1 mL.
11. Make up the volume to 1 mL with water.
12. Filter the extract through a 0.45 µm Phenex™ RC syringe filter (part no. AF0-8103-12) and then transfer to a Verex™ autosampler vial (part no. AR0-9925-13).

Note: The extracts are now ready for LC/MS/MS analysis.

SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

Florfenicol amine

Sulfanilamide

Sulfaguanidine

Tetramisole

Amoxicillin

Sulfacetamide

Lincomycin

Metronidazole

Dimetridazole

Albendazole amino sulfone

Ronidazole

Minocycline

Sulfadiazine

Thiabendazole

Sulfathiazole

Sulfapyridine

Desethylene ciprofloxacin

Ractopamine

Ofloxacin

Sulfamerazine

Enoxacin

Tetracycline

Norfloxacin

Ampicillin

Ciprofloxacin

Thiamphenicol

Lomefloxacin

Enrofloxacin

Oxytetracycline

Danofloxacin

Orbifloxacin

Difloxacin

Sarafloxacin

Sulfamethazine-d4

Sulfamethazine

2-mercaptobenzimidazole

Sulfamethizole

Ipronidazole-OH

Sulfamethoxypyridazine

Spiramycin

Chlorotetracycline

Sulfachloropyridazine

Sulfamethoxazole

Florfenicol

Sulfadoxine

Clindamycin

Tilmicosin

Sulfisoxazole

Carbadox

6-phenyl-2-thiouracil

Albendazole sulfoxide

Chloramphenicol

Doxycycline

Bacitracin

2-Quinoxalinecarboxylic acid

Oxolinic acid

Sulfadimethoxine

Albendazole sulfone

Erythomycin

Sulfaquinoloxaline

Tylosin

Fenbendazole sulfoxide

Josamycin

Fenbendazole sulfone

Albendazole

Flumequine

Sulfanitran

Ceftiofur

Mebendazole

Oxyphenbutazone

Fenbendazole

Ketoprofen

Penicillin G

Naproxen

Phenylbutazone

Flunixin

Nicarbazin

Etodolac

Dipyrone

Cloxacillin

Ibuprofen

Diclofenac

Indomethacin

Triclabendazole

Tolfenamic acid

Novobiocin

Niclosamide

Decoquinate

Sulfasalazine

Monensin

Narasin

Lasalocid

Rafoxanide