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Separation of Benzodiazepines

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Application Detail (App ID: 20047)

Column: Synergi™ 4 µm Hydro-RP 80 Å, LC Column 50 x 2 mm, Ea

Part No:00B-4375-B0

Dimensions:50 x 2 mm ID

Elution Type:Gradient

Elution A:0.1% Formic acid in DI H2O

Elution B:0.1% formic Acid in ACN

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0.5	90	10
2	0.7	80	20
3	5.9	25	75
4	6	5	95
5	6.2	5	95
6	6.3	90	10
7	8.5	90	10

Flow Rate:500 µL/min

Col. Temp: 40 °C

Detection: Tandem Mass Spec (MS-MS) @ (22°C)

Detector Info: 3200 QTRAP/API 3200

Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, AQ C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7510
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

7-Aminoclonazepam

7-Aminoflurazepam

Midazolam

Flurazepam

a-OH-Alprazolam

a-OH-Triazolam

Oxazepam

Lorazepam

Clonazepam

Nordiazepam

Alprazolam

Flunitrazepam

Temazepam

Diazepam