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MMA Calibration from Human Serum on Gemini C18, 3u, 100x3 mm

Application Detail (App ID: 20588)

Part No:00D-4439-Y0
Dimensions:100 x 3 mm ID
Elution Type:Gradient
Elution A:0.1% Formic Acid in DI H2O
Elution B:0.1% Formic Acid + 10 mM Ammonium Formate in MeOH
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 1.5 5 95
3 2.5 5 95
4 2.51 85 15
5 4.5 85 15

Flow Rate:700 µL/min
Col. Temp: 40 °C
Detection: Tandem Mass Spec (MS-MS) @ (22°C)
Detector Info: SCIEX API 4000™ (Negative Mode ESI)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Succinic acid
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2
Methylmalonic acid-d3
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3
Methylmalonic acid
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