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Nicotine and metabolites by LCMS using Gemini NX 3u C18 100x2.1mm

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Sample Preparation Details

Part Number :8B-S029-UBJ

Description :Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk

Condition 1 :2 mL Methanol

Condition 2 :2 mL Ammonium acetate buffer

Load :Load Sample

Wash 1 :2 mL Ammonium acetate buffer

Wash 2 :2 mL 30% Methanol

Dry :10 Hg for 5 min

Elution 1 :2 mL 1.5% Ammonium hydroxide methanol solution

Elution 2 :2 mL 1.5% Ammonium hydroxide methanol solution

Evaporate: :Evaporate to dryness

Reconstitute :0 µL initial mobile phase

Application Detail (App ID: 20651)

Column: Gemini® 3 µm NX-C18 110 Å, LC Column 100 x 2 mm, Ea

Part No:00D-4453-B0

Dimensions:100 x 2 mm ID

Elution Type:Gradient

Elution A:20mM Ammonium Bicarbonate

Elution B:Acetonitrile

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	90	10
2	3	25	75
3	3.1	90	10
4	5	90	10

Flow Rate:0.5 mL/min

Col. Temp: ambient

Detection: Tandem Mass Spec (MS-MS) @ (22°C)

Detector Info: SCIEX API 4000™

Analyte Details

Analyte Detail not available

Nornicotine

3-Hydroxycotinine

Anabasine

Cotinine

Nicotine