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Biogenic Amines by LCMS using Kinetex 1.7u C18 50x3.0mm

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Sample Preparation Details

Part Number :8B-S029-UBJ

Description :Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk

Condition 1 :3 mL Methanol at 1 mL/min

Condition 2 :4 mL Water

Load :Load Sample

Wash 1 :3 mL 0.1M HCl

Wash 2 :3 mL Methanol

Elution 1 :3 mL 0.2M Ammonia Solution made up in methanol

Evaporate: :Evaporate to dryness

Reconstitute :0 µL Mobile phase

Application Detail (App ID: 20685)

Column: Kinetex® 1.7 µm C18 100 Å, LC Column 50 x 3 mm, Ea

Part No:00B-4475-Y0

Dimensions:50 x 3 mm ID

Elution Type:Gradient

Elution A:30mM Ammonium formate

Elution B:Acetonitrile

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	80	20
2	3	40	60
3	3.1	0	100
4	4	0	100
5	4.1	80	20
6	5	80	20

Flow Rate:600 µL/min

Col. Temp: ambient

Detection: Tandem Mass Spec (MS-MS) @ (22°C)

Detector Info: SCIEX API 4000™

Analyte Details

Analyte Detail not available

IQx

8-MeIQx

7,8-DiMeIQx

4,8-DiMeI-Qx

MeIQ

Trp-P-2

Harman

PhIP

Norharman

AaC

MeAaC