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Heterocyclic Amines by LCMS using Kinetex 5u C18 50x2.1mm

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Sample Preparation Details

Part Number :8B-S029-UBJ

Description :Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk

Condition 1 :3 mL Methanol at 1 mL/min

Condition 2 :4 mL Water

Load :Load Sample

Wash 1 :3 mL 0.1M HCl

Wash 2 :3 mL Methanol

Elution 1 :3 mL 0.2M Ammonia Solution made up in methanol

Evaporate: :Evaporate to dryness

Reconstitute :0 µL Mobile phase

Application Detail (App ID: 20771)

Column: Kinetex® 5µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Part No:00B-4601-AN

Dimensions:50 x 2.1 mm ID

Elution Type:Gradient

Elution A:30 mM Ammonium formate

Elution B:Acetonitrile

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	95	5
2	5	40	60
3	5.1	0	100
4	6	0	100
5	7	95	5

Flow Rate:500 µL/min

Col. Temp: ambient

Detection: Tandem Mass Spec (MS-MS) @ (22°C)

Detector Info: SCIEX API 4000™

Analyte Details

Analyte Detail not available

DMIP

IQx

8-MeIQ

4,8-DiMe-IQx

7,8-DiMe-IQx

Trp-P-2

Harman

Norharman

PhIP

AaC

MeAaC

MeIQ