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Flavonoids using Luna 5um C18 250x4.6mm

Application Detail (App ID: 20801)

Part No:00G-4252-E0
Dimensions:250 x 4.6 mm ID
Elution Type:Gradient
Elution A:10mM Ammonium acetate, pH 5.0
Elution B:Acetonitrile / Methanol (9:1)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 85 15
2 20 75 25
3 30 55 45
4 35 5 95
5 37 5 95

Flow Rate:1500 mL/min
Col. Temp: 40 °C
Detection: UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detector Info: UV 270nm

Analyte Details

Analyte Detail not available

1
Quercetin
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2
Kaempferol
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3
Isorhamnetin
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