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Water-soluble Vitamins on Kinetex 2.6u, C18, 75x4.6 by HPLC (#20715)

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Application Detail (App ID: 20908)

Column: Kinetex® 2.6 µm C18 100 Å, LC Column 75 x 4.6 mm, Ea

Part No:00C-4462-E0

Dimensions:75 x 4.6 mm ID

Elution Type:Gradient

Elution A:3g Sodium 1-hexanesulfonate monohydrate/0.4mL Triethylamine/15mL Acetic acid in 1985mL H2O

Elution B:Methanol

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	95	5
2	6	53	47
3	6.1	2	98
4	7.5	2	98
5	7.51	95	5
6	11	95	5

Flow Rate:0.5 mL/min

Col. Temp: 25 °C

Detection: UV-Vis Abs.-Variable Wave.(UV) @ 270 nm (22°C)

Analyte Details

Analyte Detail not available

Ascorbic acid

Pyridoxal 5'-phosphate

Nicotinic acid

4-Pyridoxic acid

Nicotinamide

Riboflavin 5'-phosphate

Pyridoxal

Pyridoxine

Cobalamine

Folic acid

Pyridoxamine

Riboflavin

Thiamine