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Reduced Human Ig-G1 on Aeris: LC-UV Separation

Application Detail (App ID: 21521)

Part No:00D-4481-AN
Dimensions:100 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% TFA in Water
Elution B:IPA/AcCN/0.1% TFA in water 80:10:10
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 97 3
2 5 60 40
3 15 28 72
4 19 24 76
5 19.01 3 97
6 20 3 97

Flow Rate:0.4 mL/min
Col. Temp: 70 °C
Detection: UV-Vis Abs.-Diode Array (PDA) @ 214 nm (22°C)

Analyte Details

Analyte Detail not available

1
Reduced Heavy Chain Human IgG1
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2
Light Chain IgG1
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