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Human Plasma TMP & Thiamine by Gemini-NX 3u C18 100x3mm with Impact Plate_2.5nmol

Sample Preparation Details

Part Number :CE0-7565
Description :Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
Load :Load Sample
Evaporate: :Evaporate to dryness
Reconstitute :0 µL initial mobile phase

Application Detail (App ID: 21875)

Part No:00D-4453-Y0
Dimensions:100 x 3 mm ID
Elution Type:Gradient
Elution A:25nM Na2HPO4, 10% methanol
Elution B:25mM Na2HPO4, 70% methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 3 97
2 0.25 25 75
3 0.75 25 75
4 3 35 65
5 4 100 0
6 5 100 0
7 5.1 3 97
8 8 3 97

Flow Rate:0.75 mL/min
Col. Temp: ambient
Detection: Fluorescence (FLUOR) @ Ex 375 nm (22°C)
Detector Info: Shimadzu ELSD

Analyte Details

Analyte Detail not available

1
TMP
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2
Thiamine
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