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PLP and PA in Human sera/plasma on Gemini-NX 3u, C18, 100x4.6 by HPLC w Fluorescence Detection-E

Application Detail (App ID: 21888)

Part No:00D-4453-E0
Dimensions:100 x 4.6 mm ID
Elution Type:Gradient
Elution A:20mmol/L Na2HPO4 + 1mL acetic acid in 1L DI Water
Elution B:ACN : MeOH (70:30)
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 95 5
2 5 40 60
3 5.1 5 95
4 6 95 5
5 7 95 5

Flow Rate:1 mL/min
Col. Temp: 35 °C
Detection: Fluorescence (FLUOR) @ Ex 360 nm (22°C)
Detector Info: Shimadzu RF-20A

Analyte Details

Analyte Detail not available

1
Pyridoxal 5'-phosphate
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2
4-Pyridoxic acid
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3
Pyridoxal
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