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Oxytocin and ARG-Vasopressin Chromatogram

Sample Preparation Details

Part Number :8E-S035-TGB
Description :Strata-X-CW 33 µm Polymeric Weak Cation, 30 mg / well, 96-Well Plates, 2/Pk
Condition 1 :1 mL Methanol at 1 mL/min
Condition 2 :1 mL Water at 1 mL/min
Sample Pretreatment :Strata - X - CW is selected, which works for both compounds
Load :Load 300.00 µL Sample diluted in 4% H3PO4 in water
Wash 1 :1 mL 4% H3PO4 in water at 1 mL/min
Wash 2 :1 mL 30% MeOH in water at 1 mL/min
Dry :3-5 min
Elution 1 :0.75 mL 1% TFA in 50:50 MeOH: Acetonitrile at 0.75 mL/min
Elution 2 :0.75 mL 1% TFA in 50:50 MeOH: Acetonitrile at 0.75 mL/min
Evaporate: :Evaporate to dryness
Reconstitute :100 µL 1% TFA in 80:20 MPA:MPB

Application Detail (App ID: 22770)

Part No:00B-4447-B0
Dimensions:50 x 2 mm ID
Elution Type:Gradient
Elution A:0.1%FA in water
Elution B:0.1%FA in 50:50 MeOH:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 90 10
2 0.2 90 10
3 0.5 70 30
4 3.5 70 30
5 3.51 30 70
6 4 30 70
7 4.01 90 10
8 5 90 10

Flow Rate:0.45 mL/min
Col. Temp: ambient
Detection: Tandem Mass Spec (MS-MS) @ (40°C)
Detector Info: SCIEX API 5000

Analyte Details

Analyte Detail not available

1
Oxytocin
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2
ARG-Vasopressin
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