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Melamine in dog food by GC/MS using a ZB-5MSplus (EIC)

Application Detail (App ID: 23244)

Part No:7HG-G030-11
Phase:
Dimensions:30 meters x 0.25 mm x 0.25 µm
Oven Profile:75º C for 1 min to 320 ºC at 15 ºC/min hold for 4 min
Carrier Gas:Constant Flow Helium, 1 mL/min
Injection:Splitless :1 1 µL @ 280°C
Detection: Mass Selective (MSD) (320°C)
Note:Sample preparation: Combine 0.5 g of homogenized dog food with 10 mL of DEA/water/acetonitrile (1:4:5) in a 15 mL centrifuge tube. Sonicate for 30 min. Centrifuge at 5000 rpm for 10 min. Transfer 100 µL of supernatant to an autosampler vial and evaporate to dryness using nitrogen gas. Reconstitute with 100 µL of acetonitrile/pyridine (1:1) and then derivatize using 100 µL of BSTFA with 1% TCMS at 70 ºC for 45 min. Inject sample into the GC/MS.

Dry dog food spiked with 100 µg/g melamine and related compounds.

Sim mode: Cyanuric acid (345,330,346), Ammelide (344,329,345), Ammeline (328,343,329), Melamine (327,342,328), Benzoguanamine (316,331,332)

Analyte Details

Analyte Detail not available

1
Cyanuric acid
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2
Ammelide
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3
Ammeline
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4
Melamine
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5
Benzoguanamine
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