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Applications

FAMEs In Palm Oil by GC/FID on Zebron ZB-FAME

Sample Preparation Details

Part Number :8B-S012-KDG
Description :Strata SI-1 Silica (55 µm, 70 Å), 2 g / 12 mL, Giga Tubes, 20/Pk
Condition 1 :6 mL Hexane
Load :Load 3.80 mL Sample diluted in Pretreated Sample
Elution 1 :5 mL Hexane/Ethyl Acetate (87:13)
Evaporate: :Evaporate to dryness
Reconstitute :400 µL Hexane
Notes :To reconstituted sample, add 200 µL of 2 M potassium hydroxide in methanol, cap tube and vortex. Wat 5 minutes. Add 2 mL Milli-Q® water, vortex. Allow solution to settle then transfer top layer to Q-sert vial for GC analysis.

Application Detail (App ID: 23969)

Part No:7KG-G033-10
Phase:
Dimensions:60 meters x 0.25 mm x 0.2 µm
Oven Profile:180 °C isothermal
Carrier Gas:Constant Flow Helium, 1.2 mL/min
Injection:Split 100:1 1 µL @ 240°C
Detection: Flame Ionization (FID) (240°C)
Note:Recommended Accessories:
Recommended Liner: Zebron PLUS Single Taper with Wool, 4 mm ID
Liner Part No.: AG2-0A11-05 (for Agilent systems)
Inlet Seal: AG0-8620 (Gold Plated Easy Seal)
Septum: AG0-4696 (PhenoRed-400)

Sample Preparation:
1. Weigh 0.2 g of sample into a glass test tube
2. Add 3.8 mL of hexane and vortex
3. Condition SPE tube (Strata® Si-1, 2 g/12 mL, Part No.: 8B-S012-KDG) under vacuum with 6 mL of hexane; do not let bed go dry
4. Load sample with light vacuum
5. Elute with 5 mL of hexane:ethyl acetate (87:13)
6. Evaporate under nitrogen at 35 °C
7. Reconstitute with 4 mL of hexane
8. Add 200 µL of 2 M potassium hydroxide/methanol solution and vortex
9. Leave at room temperature for 5 minutes
10. Add 2 mL of deionized water and vortex
11. Leave to separate until upper layer becomes clear
12. Extract upper layer for GC analysis

Analyte Details

Analyte Detail not available

1
C12:0
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2
C14:0
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3
C16:0
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4
C16:1 cis 9
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5
C18:0
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6
C18:1 cis 9
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7
C18:2 cis 9,12
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8
C18:3 cis 9,12,15
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9
C20:0
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10
C20:1 cis 11
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