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IntantPC Labeled N-Linked Glycans on Trastuzumab, Innovator on bioZen Glycan

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Application Detail (App ID: 25039)

Column: bioZen™ 2.6 µm Glycan , LC Column 150 x 2.1 mm, Ea

Part No:00F-4773-AN

Dimensions:150 x 2.1 mm ID

Elution Type:Gradient

Elution A:100 mM Ammonium Formate, pH 4.5

Elution B:Acetonitrile

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	22	78
2	10	25.5	74.5
3	24	28	72
4	38.5	44.1	55.9
5	38.6	60	40
6	40.6	60	40
7	40.7	22	78
8	48	22	78

Flow Rate:0.5 mL/min

Col. Temp: 50 °C

Detection: LC/FLD (Fluorescence Detector) @ 285.0000000000 nm (nanometers) (0°C)

Note:Utilize bioZen N-Glycan Clean-Up microelution SPE 96well plate for sample preparation (Part No.: 8M-S009-NGA).

Analyte Details

Analyte Detail not available

G0-GlcNAc

G0F-GlcNAc

Man5

G0F

G1F-GlcNAc

G1F

G1F'

G2F

Sialylated Glycan