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Extraction of Vet Drugs in Milk Using Strata-X PRO with a Kinetex 2.6 µm Biphenyl 50 x 3.0mm

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Sample Preparation Details

Part Number :8B-S536-UBJ

Description :Strata-X PRO

Sample Pretreatment :To 1 mL of milk add 3 mL of 0.2% formic acid in Acetonitrile /Methanol (90:10). Mix/vortex for 15-20 secs. Centrifuge for 5 min at 10,000 rpm. Collect supernatant to perform SPE in next step.

Load :Supernatant (from the pre-treatment) through cartridge and collect

Evaporate: :Evaporate to dryness

Reconstitute :Dried sample in 1 mL of 0.1% Formic acid in Water/ 0.1% Formic acid in Acetonitrile (95:5)

Load	Supernatant (from the pre-treatment) through cartridge and collect
Dry Down	Sample under a gentle stream of Nitrogen
Reconstitute	Dried sample in 1 mL of 0.1% Formic acid in Water/ 0.1% Formic acid in Acetonitrile (95:5)

Application Detail (App ID: 25487)

Column: Kinetex® 2.6 µm Biphenyl 100 Å, LC Column 50 x 3.0 mm, Ea

Part No:00B-4622-Y0

Dimensions:50 x 3 mm ID

Elution Type:Gradient

Elution A:0.1%Formic acid in Water

Elution B:0.1%Formic acid in Methanol

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	95	5
2	1.5	5	95
3	3	5	95
4	3.01	95	5
5	4.5	95	5

Flow Rate:0.5 mL/min

Col. Temp: 45 °C

Detection: LC/MS/MS @ 210.0000000000 nm (nanometers) (30°C)

Note:ALTA DENA 2% Reduced Fat Milk With initial mobile phase

Analyte Details

Analyte Detail not available

Sulfaguanidine

Lincomycin

Sulfadiazine

Cephapirin

Sulfamerazine

Sulfamethoxazole

Sulfamethizole

Cefalexin

Sulfamethazine

Cortisone

Cortisol

β-methasone

Prednisolone