Region

Applications

Removal of Phospholipids from Milk Sample Using Strata-X PRO With a Kinetex 2.6 µm C18 50 x 2.1 mm

Sample Preparation Details

Part Number :8B-S536-UBJ
Description :Strata-X PRO
Sample Pretreatment :To 1 mL of milk add 3 mL of 0.2% formic acid in Acetonitrile /Methanol (90:10). Mix/vortex for 15-20 secs. Centrifuge for 5 min at 10,000 rpm. Collect supernatant to perform SPE in next step.
Load :Supernatant (from the pre-treatment) through cartridge and collect
Evaporate: :Evaporate to dryness
Reconstitute :Dried sample in 1 mL of 0.1% Formic acid in Water/ 0.1% Formic acid in Acetonitrile (95:5)
Load Supernatant (from the pre-treatment) through cartridge and collect
Dry Down Sample under a gentle stream of Nitrogen
Reconstitute Dried sample in 1 mL of 0.1% Formic acid in Water/ 0.1% Formic acid in Acetonitrile (95:5)

Application Detail (App ID: 25488)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1%Formic acid in Water
Elution B:0.1%Formic acid in Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 60 40
2 0.5 5 95
3 11.5 5 95
4 11.51 60 40
5 13 60 40

Flow Rate:0.4 mL/min
Col. Temp: 45 °C
Detection: LC/MS/MS @ 0.0000000000 nm (nanometers) (30°C)
Note:2% liquid Milk (ALTA DENA DairyPure) utilized for sample prep extraction Analyte diluted with initial mobile phase

Analyte Details

Analyte Detail not available

1
Lyso PC
search

2
PC-1
search

3
PC-2
search