Region

Applications

Water-Soluble Vitamins

Application Detail (App ID: 2553)

Part No:00F-4251-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:Methanol
Elution B:0.005 M Hexanesulfonic Acid (0.1% TFA and 0.01% TEA) with 5 % Methanol premixed
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 0 100
2 5 0 100
3 16 23 77
4 26 26 74
5 28 50 50
6 30 0 100
7 45 0 100

Flow Rate:0.55 mL/min
Col. Temp: 30 °C
Detection: UV-Vis Abs.-Diode Array (PDA) @ 260/280 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C18 4 x 3.0mm, 10/Pk Part No.: AJ0-4287
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available

1
Ascorbic acid
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2
Niacin
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3
Pyridoxal 5'-phosphate
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4
Niacinamide
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5
Paraminobenzoic acid
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6
Riboflavin 5'-phosphate
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7
Pyridoxine
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8
Folic acid
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9
Cyanocobalamin
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10
Thiamine
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11
Riboflavin
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