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LC-MS/MS Chromatogram for Amlodipine Enantiomers and Internal Standards

Sample Preparation Details

Part Number :8B-S100-TAK
Description :Strata™-X 33 µm Polymeric Reversed Phase
Condition 1 :1 mL Methanol
Load :Spiked Plasma
Wash 1 :1 mL Water
Wash 2 :1 mL 20% Methanol in Water
Elution 1 :1 mL of 0.1% Formic Acid in Methanol
Evaporate: :Evaporate to dryness
Reconstitute :100 µL of Mobile Phase
Condition 1 mL Methanol
Condition 1 mL Methanol
Wash 1 mL 20% Methanol in Water
Wash 1 mL Water
Wash 1 mL 20% Methanol in Water
Elute 1 mL of 0.1% Formic Acid in Methanol
Dry Down Take to dryness under a gentle stream of Nitrogen at 50 °C
Reconstitute 100 µL of Mobile Phase
Notes :5 µL of internal standard working solution was added to 100 µL spiked human plasma (spiked at 0.050, 0.150, 20.0, 40.0, and 50.0 ng/mL) and mixed, 500 µL of 0.2% (v/v) Ethanolamine in water was added to sample and vortexed.

Application Detail (App ID: 25531)

Part No:00F-4490-B0
Dimensions:150 x 2 mm ID
Elution Type:Gradient
Elution A:0.05% Ethanolamine in Acetonitrile and Isopropyl Alcohol (96:4 v/v)
Gradient Profile:
Step No. Time (min) Pct A
1 0 100
2 4 100

Flow Rate:0.3 mL/min
Col. Temp: 25 °C
Detection: LC/MS/MS @ 0.0000000000 (0°C)

Analyte Details

Analyte Detail not available

1
(R)-Amlodipine
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2
(R)-Amlodipine-d4
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3
(S)-Amlodipine
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4
(S)-Amlodipine-d4
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