| Dispense |
25 µL of bead slurry into a 96 well plate |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Reconstitute |
beads with PBS buffer to the original slurry volume. |
| Vortex |
bead slurry using mixer or pipette |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Add |
500µL PBS Buffer to each plate well that contains beads |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Vortex |
bead slurry using mixer or pipette |
| Transfer |
50 µL of bead slurry into each well that contains 25 µL sample. Mix well before transferring |
| Reconstitute |
beads with PBS buffer to the original slurry volume. |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Transfer |
plate for incubation for one hour at room temperature with a shaking speed of 1200 RPM using a deep well plate thermoshaker. Beads should stay suspended during incubation |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Add |
500µL PBS Buffer to each plate well that contains beads |
| Transfer |
96 well plate onto magnetic stand for 5 minutes and collect supernatant |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Add |
500µL PBS Buffer to each plate well that contains beads |
| Shake |
and mix the bead slurry, using vortex or pipette |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Add |
500µL PBS Buffer to each plate well that contains beads |
| Shake |
and mix the bead slurry, using vortex or pipette |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Reconstitute |
beads with PBS buffer to the original slurry volume. |
| Shake |
and mix slurry of anti-human IgG coated beads well, using vortex or pipette |
| Transfer |
50 µL of bead slurry into each well that contains 25 µL sample. Mix well before transferring |
| Add |
cover to plate using polyester plate film |
| Vortex |
to mix plate |
| Transfer |
plate for incubation for one hour at room temperature with a shaking speed of 1200 RPM using a deep well plate thermoshaker. Beads should stay suspended during incubation |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Add |
200 µL of PBS Buffer to each well that contains beads |
| Vortex |
well and place plate on centrifuge for 2 minutes at 400RPM |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Add |
200µL of 10mM Ammonium Bicarbonate to each well that contains beads |
| Vortex |
well and place plate on centrifuge for 2 minutes at 400RPM |
| Transfer |
96 well plate onto magnetic stand for 30 seconds and discard liquid |
| Add |
100µL of 0.1% TFA in water to each well and vortex |
| Add |
pH paper to check pH value (should be lower than 3) |
| Add |
plate cover and shake for 10 minutes at 1200RPM using thermomixer |
| Transfer |
plate on centrifuge for 2 minutes at 400RPM |
| Transfer |
96 well plate onto magnetic stand for 5 minutes and collect supernatant |
