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Clean-Up of Phospholipids in Human Plasma Comparing PPT VS. Strata-X PRO SPE on a Kinetex 2.6 µm C18 50 x 2.1 mm

Sample Preparation Details

Part Number :8E-S536-TGA
Description :Strata-X PRO
Condition 1 :1 mL of ACN
Sample Pretreatment :Sample Pre-treatment: • Add 100 µL Serum to tube • Add 900 µL of chilled (~0°C) ACN to the serum while vortexing • Centrifuge samples at 6000 rpm for 5 minutes and transfer supernatant
Load :supernatant (collected from pre-treatment)
Evaporate: :Evaporate to dryness
Reconstitute :100 µL of initial mobile phase
Condition 1 mL of ACN
Load supernatant (collected from pre-treatment)
Vacuum Apply and collect extract
Dry Down Sample under N2 and heat at 45 °C
Reconstitute 100 µL of initial mobile phase

Application Detail (App ID: 25779)

Part No:00B-4462-AN
Dimensions:50 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% Formic acid in water
Elution B:0.1% Formic acid in methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 60 40
2 0.5 5 95
3 11.5 5 95
4 11.51 60 40
5 13.5 60 40

Flow Rate:400 μL/min
Col. Temp: 40 °C
Detection: LC/MS/MS @ (40°C)
Note:Analyte dilution not needed (Direct injection of the extracted sample)

Analyte Details

Analyte Detail not available

1
Lyso PC
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2
PC-1
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3
PC-2
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