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Reversed Phase Analysis of Reduced NIST mAb-Temperature Effect (Detailed Overlay of Heavy Chain)

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Application Detail (App ID: 25820)

Column: bioZen™ 2.6 µm WidePore C4, LC Column 100 x 2.1 mm, Ea

Part No:00D-4786-AN

Dimensions:100 x 2.1 mm ID

Elution Type:Gradient

Elution A:0.1% TFA in Water

Elution B:0.1% TFA in Acetonitrile

Gradient Profile:

Step No.	Time (min)	Pct A	Pct B
1	0	75	25
2	6	55	45
3	6.1	10	90
4	6.3	80	20
5	6.5	10	90
6	6.7	80	20
7	6.9	10	90
8	7.1	80	20
9	8	80	20

Flow Rate:0.5 mL/min

Col. Temp: 70 °C

Detection: LC/UV (DAD, PDA) @ 214.0000000000 nm (nanometers) (0°C)

Analyte Details

Analyte Detail not available

Step No.	Time (min)	Pct A	Pct B
1	0	75	25
2	6	55	45
3	6.1	10	90
4	6.3	80	20
5	6.5	10	90
6	6.7	80	20
7	6.9	10	90
8	7.1	80	20
9	8	80	20

Step No.	Time (min)	Pct A	Pct B
1	0	75	25
2	6	55	45
3	6.1	10	90
4	6.3	80	20
5	6.5	10	90
6	6.7	80	20
7	6.9	10	90
8	7.1	80	20
9	8	80	20

Step No.	Time (min)	Pct A	Pct B
1	0	75	25
2	6	55	45
3	6.1	10	90
4	6.3	80	20
5	6.5	10	90
6	6.7	80	20
7	6.9	10	90
8	7.1	80	20
9	8	80	20