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Intact Reversed Phase Analysis of NIST mAb-Effect of Flow Rate

Application Detail (App ID: 25821)

Part No:00D-4786-AN
Dimensions:100 x 2.1 mm ID
Elution Type:Gradient
Elution A:0.1% TFA in Water
Elution B:0.1% TFA in Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 75 25
2 6 55 45
3 6.1 10 90
4 6.3 80 20
5 6.5 10 90
6 6.7 80 20
7 6.9 10 90
8 7.1 80 20
9 8 80 20

Flow Rate:0.5 mL/min
Col. Temp: 90 °C
Detection: LC/UV (DAD, PDA) @ 280.0000000000 nm (nanometers) (0°C)

Analyte Details

Analyte Detail not available