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Sildenafil Citrate and Sildenafil N-Oxide USP System Suitability Solution on Kinetex 5 µm C18 150x4.6mm LC/UV

Application Detail (App ID: 26206)

Part No:00F-4601-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:Buffer (dilute 7 mL of triethylamine with water to 1 L. Stir and adjust with phosphoric acid to a pH of 3.0+/- 0.1), Methanol and Acetonitrile (58:25:17)
Gradient Profile:
Step No. Time (min) Pct A
1 20 100

Flow Rate:1.4 mL/min
Col. Temp: 30 °C
Detection: LC/UV (DAD, PDA) @ 290.0000000000 nm (nanometers) (30°C)
Note:Dissolve 70 mg of Sildenafil Citrate in 1 mL of a solution of hydrogen peroxide and anhydrous formic acid (2:1). Allow to stand for at least 10 minutes to generate Sildenafil N-Oxide, then dilute w/mobile phase to 250 mL.

Analyte Details

Analyte Detail not available

1
Sildenafil citrate
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2
Sildenafil N-Oxide
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