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Levothyroxine and Impurity A according to Ph.Eur. Monograph 0401 on Luna Omega 3 µm C18 150 x 4.6 mm @1.32 mL/min

Application Detail (App ID: 26227)

Part No:00F-4784-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:Dissolve 1.97 g of phosphoric acid in water and dilute to 2 L with water
Elution B:Dissolve 1.97 g of phosphoric acid in acetonitrile and dilute to 2 L with acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 70 30
2 10 70 30
3 40 20 80
4 50 20 80

Flow Rate:1.32 mL/min
Col. Temp: 25 °C
Detection: LC/UV (DAD, PDA) @ 225.0000000000 nm (nanometers) (0°C)

Analyte Details

Analyte Detail not available

1
Impurity A
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2
Levothyroxine
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