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Hydrocortisone Extraction from Topical Cream Unfiltered with Guard

Application Detail (App ID: 26462)

Part No:00F-4252-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Gradient
Elution A:Water
Elution B:Methanol
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 50 50
2 20 50 50

Flow Rate:1 mL/min
Col. Temp: 35 °C
Detection: LC/UV (DAD, PDA) @ 220.0000000000 nm (nanometers) (0°C)
Note:Hydrocortisone cream 1. Dissolve 1g of Hydrocortisone cream in 10mL ethyl acetate (n=6)
2. Dilute 2mL of ethyl acetate-cream sample with 8mL methanol:water (1:1)
3. Load 400uL methanol:water (1:1) cream sample
4. Push sample through filter vial
5. Analyze final eluate via HPLC-UV

Analyte Details

Analyte Detail not available